Comparative Evaluation of in-vitro Antioxidant Capacity of Petroleum-ether Extract of Hibiscus sabdarrifa (Roselle Flower) and Jatropha tanjorensis Leaf
Akpata E. I. *
Department of Applied Biochemistry, Faculty of Biological Sciences, Enugu State University of Science and Technology, Enugu, Nigeria.
Ani O. N.
Department of Applied Biochemistry, Faculty of Biological Sciences, Enugu State University of Science and Technology, Enugu, Nigeria.
Achikanu C. E.
Department of Applied Biochemistry, Faculty of Biological Sciences, Enugu State University of Science and Technology, Enugu, Nigeria.
*Author to whom correspondence should be addressed.
Abstract
Aims: The comparative evaluation of in vitro antioxidant capacity of petroleum-ether extract of Hibiscus sabdarrifa (Roselle flower) and Jatropha tanjorensis leaf was carried out in this study.
Methodology: This was done using various antioxidant assays such as Ferric Reducing Antioxidant Power, 2, 2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid, 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity, Lipid Peroxidation and Nitric Oxide Radical scavenging activity. Standard biochemical methods were used for the analyses. Ascorbic acid served as the standard antioxidant.
Results: The result showed a non-significant increase (P >0.05) in the Ferric reducing power capacity and 2, 2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid of Jatropha tanjorensis leaf when compared to Hibiscus sabdarrifa (Roselle flower). There was a significant (P < 0.05) increase in free radical scavenging activity of Jatropha tanjorensis leaf extract when compared to Hibiscus sabdarrifa (Roselle flower). The result also showed a significant (P < 0.05) increase in lipid peroxidation scavenging activities of Jatropha tanjorensis leaf when compared to Hibiscus sabdarrifa (Roselle flower). There was a non-significant (P > 0.05) decrease in Nitric oxide scavenging activities of Hibiscus sabdarrifa (Roselle flower) and Jatropha tanjorensis leaf extract when compared to the standard antioxidant L-ascorbic acid.
Conclusion: This study showed that although Hibiscus sabdariffa possesses antioxidant capacity, Jatropha tanjorensis leaf extract had a higher antioxidant activity.
Keywords: Antioxidant, free radicals, comparative, Hibiscus sabdarrifa, Jatropha tanjorensis