Isolation and Characterisation of Hydrolysable Tannin from Ethyl Acetate Portion of the Aerial Part of Phyllanthus amarus Schum. & Thonn
Halima A. Umar *
Department of Chemistry, University of Maiduguri, P.M.B. 1069, Maiduguri, Nigeria.
Hamidu Usman
Department of Chemistry, University of Maiduguri, P.M.B. 1069, Maiduguri, Nigeria.
Mustapha B. Abubakar
Department of Veterinary Microbiology, University of Maiduguri, P.M.B. 1069, Maiduguri, Nigeria.
Baba F. Mohammed
Department of Chemistry, University of Maiduguri, P.M.B. 1069, Maiduguri, Nigeria.
Mohammed Babakura
Department of Chemistry, University of Maiduguri, P.M.B. 1069, Maiduguri, Nigeria.
Mohammed A. Sule
Department of Chemistry, University of Maiduguri, P.M.B. 1069, Maiduguri, Nigeria.
*Author to whom correspondence should be addressed.
Abstract
The present work involves extraction of phytochemicals from aerial part of Phyllanthus amarus Schum. & Thonn with n-hexane and 85% methanol. The isolation and characterization of Phytoconstituents was done from the methanol extract through portioning with chloroform and ethyl acetate. Fractionation and isolation (using column and thin layer chromatography respectively) of ethyl acetate column pooled portion afforded a compound coded as E-3.3C. The structure of the isolated compound was established on spectroscopic evidences (IR, 1HNMR, MS), which revealed the compound as 1-de (oxygalloyl)-2ˈ,3ˈ,-di-methoxy-amariin a hydrolysable tannins.
Keywords: Phyllanthus amarus, fractionation, fragmentation, soxhlet extraction, spectroscopy