Development and Validation of Ultra Visible Spectrophotometric Method for the Estimation of Thymoquinone
Sagar Trivedi
*
Department of Pharmaceutics and Pharmaceutical Sciences, Rashtrasant Tukadoji Maharaj Nagpur University Campus, Nagpur, India.
Veena Belgamwar
Department of Pharmaceutics and Pharmaceutical Sciences, Rashtrasant Tukadoji Maharaj Nagpur University Campus, Nagpur, India.
Kamlesh Wadher
Department of Pharmaceutics, Smt. Kishoritai Bhoyar College of Pharmacy, Kamptee, Nagpur, Maharashtra, 441002, India.
*Author to whom correspondence should be addressed.
Abstract
Aim: The work mainly focuses on developing a robust UV-Visible spectroscopic method for qualitative and quantitative analysis of thymoquinone and this will open to many possibilities in exploiting the wonders of this bioactive molecule.
Study Design: UV-vis Spectrophotometric method development for thymoquinone.
Place and Duration of Study: Department of Pharmaceutics, Department of Pharmaceutical Sciences, Rashtrasant Tukadoji Maharaj Nagpur University Campus, Nagpur, India.
Methodology: To find out λmax and validate the developed method using Double beam UV – visible spectrophotometer module (JASCO V-630).
Results: The thymoquinone was found to be soluble in methanol. The absorption maximum (λmax) was found to be 252 nm. The good linearity was found to be within concentration range of 2-10 μg/ml with correlation coefficient (r2) >0.99 and regression equation of the curve was found to be y = 0.0173x − 0.0394 at 252 nm. The precision (intra-day and inter-day) data represents good reproducibility with % RSD lower than 2.0% which assured that method is précised. Mean recovery value at different concentrations was found to be higher than 90%, indicates accuracy of the method. LOD and LOQ for thymoquinone were reported and were found to be 0.016 μg/ml and 0.0531μg/ ml 252 nm.
Conclusion: The developed method was found to be simple, specific, economic, reliable, accurate, precise, and reproducible used as a quality control tool for analysis of pure thymoquinone and thymoquinone in formulations.
Keywords: Thymoquinone, UV-vis spectrophotometric method, validation, accuracy, limit of detection, limit of quantification