Chemical Variability of Essential Oils from Zanthoxylum leprieurii Guill. et Perr (Rutaceae) According to Plant Organs

Main Article Content

Yoro Tine
Alioune Diallo
William Diatta
Jean-Marie Desjobert
Jean Costa
Alassane Wélé
Julien Paolini

Abstract

Zanthoxylum leprieurii belonging to Rutaceae family, is a medicinal and aromatic plants widely used in ethnopharmacology. Like other plants of Rutaceae family such as Citrus sp., the essential oils of this species could be used in cosmetic and pharmaceutical products. Thus, the essential oils of Z. leprieurii from different plant organs (fruit, leaf, stem and root) were investigated by using GC and GC-MS. The volatile composition of Z. leprieurii fruits exhibited relative high amounts of hydrocarbons monoterpenes (90.9%) such as (E)-β-ocimene (50.9%) and a-pinene (30.4%). The chemical composition of fruit oils was compared with volatile fractions of leaves, stems and roots from the same plant station. Germacrene B (9.0%), b-phellandrene (7.6%), caryophyllene oxide (7.2%) and b-caryophyllene (5.3%) were identified as the major constituents of leaves whereas the essential oil composition of the roots dominated by sesquiterpenes (83.3%) such as germacrone (23.5%), germacrene B (19.1%), g-elemene (6.0%), elemenone (4.0%) and b-elemene (2.4%) in the essential oil of the stems, b-Phellandrene (12.7%), germacrene B (5.0%), germacrene D (4.7%) and cis-9-Octadecen-1-ol (4.6%) have been reported as majority compounds.

Keywords:
Essential oils, Zanthoxylum leprieurii, GC-MS.

Article Details

How to Cite
Tine, Y., Diallo, A., Diatta, W., Desjobert, J.-M., Costa, J., Wélé, A., & Paolini, J. (2020). Chemical Variability of Essential Oils from Zanthoxylum leprieurii Guill. et Perr (Rutaceae) According to Plant Organs. Asian Journal of Applied Chemistry Research, 5(1), 18-25. https://doi.org/10.9734/ajacr/2020/v5i130124
Section
Original Research Article

References

Tabuti JRS. Zanthoxylum leprieurii Guill. Perr. In: Schmelzer GH. & Gurib-Fakim, A. PROTA (Plant Resources of Tropical Africa): Wageningen, Netherlands; 2011.

Lamorde M, Tabuti JR, Obua C, Kukunda-Byobona C, Lanyero H, Byakika-Kibwika P. Medicinal plants used by traditional medicine practitioners for the treatment of HIV / AIDS and related conditions in Uganda. J Ethnopharmacol. 2010;130(1): 43-53.

DOI:https://doi.org/10.1016/j.jep.2010.04.004

Kerharo J, Adam JG. The traditional Senegalese pharmacopoeia; Vigot Frères: Paris; 1974.

Noumi E. Spice, condiment and flavoring plants from Cameroon. Doctoral thesis. University of Yaoundé; 1984.

Tatsadjieu LN, Essia Ngang JJ, Ngassoum MB, Etoa FX. Antibacterial and antifungal activity of Xylopia aethiopica, Monodora myristica, Zanthoxylum xanthoxyloides and Zanthoxylum leprieurii from Cameroon. Phytotherapy. 2003;74(5):469-472.
DOI:https://doi.org/10.1016/S0367-326X(03)00067-4

Gardini F, Belletti N, Ndagijimana M, Guerzoni ME, Tchoumbougnang F, Zollo PHA et al. Composition of four essential oils obtained from plants from Cameroon, and their bactericidal and bacteriostatic activity against Listeria monocytogenes, Salmonella enteritidis and Staphylococcus aureus. Afr J Microbiol Res. 2009;3(5): 264-271.

Misra LN, Wouatsa NV, Kumar S, Kumar RV, Tchoumbougnang F. Antibacterial, cytotoxic activities and chemical composition of fruits of two Cameroonian Zanthoxylum species. J Ethnopharmacol. 2013;148(1):74–80.
DOI:https://doi.org/10.1016/j.jep.2013.03.069

Talontsi FM, Douanla-Meli C, Laatsch H. Depsidones from an endophytic fungus Chaetomium sp. Associated with Zanthoxylum leprieurii. Zeitschrift für Naturforschung B. 2014;68(11):1259–1264.
DOI:https://doi.org/10.5560/znb.2013-3168

Ngane AN, Biyiti L, Zollo PA, Bouchet PH. Evaluation of antifungal activity of extracts of two Cameroonian Rutaceae: Zanthoxylum leprieurii Guill. et Perr. and Zanthoxylum xanthoxyloides Waterm. J Ethnopharmacol. 2000;70(3):335–342.
DOI:https://doi.org/10.1016/S0378-8741(99)00188-9

Matasyoh JC, Talontsi FM, Ngoumfo MR, Chepkorir R. Larvicidal activity of acridone alkaloids from Zanthoxylum leprieurii against the malaria vector Anopheles gambiae. Nat Prod J. 2011;1(2):121–124.

Tchinda AT, Fuendjiep V, Sajjad A, Matchawe C, Wafo P, Khan S et al. Bioactive compounds from the fruits of Zanthoxylum leprieurii. Pharmacology-online. 2009;1:406–415.

Ngoumfo RM, Jouda JB, Mouafo FT, Komguem J, Mbazoa CD, Shiao TC et al. In vitro cytotoxic activity of isolated acridones alkaloids from Zanthoxylum leprieurii Guill. et Perr. Bioorg. Med. Chem. 2010;18(10):3601–3605. DOI:https://doi.org/10.1016/j.bmc.2010.03.040

Choumessi AT, Loureiro R, Silva AM, Moreira AC, Pieme, AC, Tazoacha A, et al. Toxicity evaluation of some traditional African spices on breast cancer cells and isolated rat hepatic mitochondria. Food Chem Toxicol. 2012;50(11):4199–4208.
DOI:https://doi.org/10.1016/j.fct.2012.08.008

Dongmo PM, Tchoumbougnang F, Sonwa ET, Kenfack SM, Zollo PH, Menut C. Antioxidant and anti-inflammatory potential of essential oils of some Zanthoxylum (Rutaceae) of Cameroon. Int J Essen Oil Ther. 2008;2(2):82–88.

Bouba A, Njintang YN, Scher J, Mbofung CMF. Phenolic compounds and radical scavenging potential of twenty Cameroonian spices. Agric Biol J North Am. 2010;1(3);213–224.

Womeni HM, Djikeng FT, Tiencheu B, Linder M. Antioxidant potential of methanolic extracts and powders of some Cameroonian spices during accelerated storage of soybean oil. Adv Biol Chem. 2013;03(03):304-10. DOI:10.4236/abc.2013.33034

Guetchueng ST, Nahar L, Ritchie KJ, Ismail FMD, Wansi JD, Evans A et al. Kaurane Diterpenes from the Fruits of Zanthoxylum leprieurii (Rutaceae). Rec Nat Prod. 2017;11(3):304–309.

Calderwood JM, Fish F. Screening for tertiary and quaternary alkaloids in some African Fagara species. J Pharm Pharmacol. 1966;18(S1):119S-125S.

Fonzes L, Winternitz F. Study of the alkaloids of Fagara léprieurii, Engl. II. Phytochemistry, 1968;7 (10):1889–1890.

Fish F, Waterman PG. Alkaloids from two Nigerian species of Fagara. J Pharm Pharmacol. 1971;23:132S-135S.

Waterman PG, Gray AI, Crichton EG. A comparative study on the alkaloids of Zanthoxylum leprieurii, Z. lemairei and Z. rubescens from Ghana. Biochem Syst Ecol. 1976;4(4):259–262.

Adesina SK. The Nigerian Zanthoxylum; chemical and biological values. Afr J Tradit Complement Altern Med. 2005;2(3):282–301.

Reisch J, Adesina SK, Bergenthal D. Constituents of Zanthoxylum leprieurii fruit pericarps. CIII: Natural product chemistry. Pharmazie. 1985;40(11):811–812.

Ekundayo O, Oguntimein B, Adeoye A. Volatile constituents of the essential oil of Zanthoxylum leprieurii. Fitoterapia. 1986; 57(4):267–269.

Lamaty G, Menut C, Bessiere JM, Aknin M. Aromatic plants of tropical Central Africa. II. A comparative study of the volatile constituents of Zanthoxylum leprieurii (Guill. et Perr.) Engl. and Zanthoxylum tessmannii Engl. leaves and fruit pericarps from the cameroon. Flavour Fragr J. 1989;4(4):203–205.
DOI:https://doi.org/10.1002/ffj.2730040411

Fogang HPD, Tapondjou LA, Womeni HM, Quassinti L, Bramucci M, Vitali LA et al. Characterization and biological activity of essential oils from fruits of Zanthoxylum xanthoxyloides Lam. and Z. leprieurii Guill. & Perr., two culinary plants from Cameroon. Flavour Fragr J. 2012;27(2): 171–179.
DOI: https://doi.org/10.1002/ffj.3083

Oyedeji O, Lawal OA, Adeniyi BA, Alaka SA, Tetede E. Essential oil composition of three Zanthoxylum Species. Journal of Essential Oil Research. 2008;20(1):69–71.
DOI:https://doi.org/10.1080/10412905.2008.9699425

Council of Europe. European Pharmacopoeia. 3rd Ed. Council of Europe: Strasbourg; 1997.

NIST (National Institute of Standards and Technology), N. PC Version of the NIST/EPA/NIH Mass Spectra Library. 4th ed. Wiley; 2008.

König WA, Joulain D, Hochmuth DH. Terpenoids and related constituents of essential oils. Library of MassFinder 3. Hochmuth DH (Ed.). Convenient and rapid analysis of GC-MS. Incorporating W.A: Hamburg; 2004.

Adams RP. Identification of essential oil components by gas chromatography/ mass spectrometry. 4th Ed. Allured publishing corporation: Carol Stream, Illinois; 2007.